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	<title>amateurmycology.com&#187; Mycorestoration</title>
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		<title>Working with Pine Loving Pleurotus</title>
		<link>http://amateurmycology.com/?p=1164</link>
		<comments>http://amateurmycology.com/?p=1164#comments</comments>
		<pubDate>Tue, 20 Mar 2012 09:05:30 +0000</pubDate>
		<dc:creator>Graham</dc:creator>
				<category><![CDATA[Featured Publications]]></category>
		<category><![CDATA[In the Lab]]></category>
		<category><![CDATA[Mycorestoration]]></category>
		<category><![CDATA[Pleurotus/ Oysters]]></category>
		<category><![CDATA[amateur]]></category>
		<category><![CDATA[Amateur Mycology]]></category>
		<category><![CDATA[beetle kill]]></category>
		<category><![CDATA[colorado]]></category>
		<category><![CDATA[conifer]]></category>
		<category><![CDATA[DNA]]></category>
		<category><![CDATA[Dr. Rytas Vilgalys]]></category>
		<category><![CDATA[Duke]]></category>
		<category><![CDATA[habitats]]></category>
		<category><![CDATA[honey mushroom]]></category>
		<category><![CDATA[ITS]]></category>
		<category><![CDATA[mycelium running]]></category>
		<category><![CDATA[mycoforestry]]></category>
		<category><![CDATA[mycology]]></category>
		<category><![CDATA[oyster]]></category>
		<category><![CDATA[Paul Stamets]]></category>
		<category><![CDATA[PCR]]></category>
		<category><![CDATA[pine beetle]]></category>
		<category><![CDATA[pine loving]]></category>
		<category><![CDATA[pleurotus]]></category>
		<category><![CDATA[pleurotus populinus]]></category>
		<category><![CDATA[pleurotus pulomnarius]]></category>
		<category><![CDATA[Rocky Mountain]]></category>
		<category><![CDATA[sequence]]></category>
		<category><![CDATA[soil depth]]></category>

		<guid isPermaLink="false">http://amateurmycology.com/?p=1164</guid>
		<description><![CDATA[This quick update will cover our recent project with Dr. Rytas Vilgalys, Professor of Biology at Duke University. Dr. Rytas was kind enough to do some PCR DNA and get an ITS sequence of some of our various collections of Pleurotus that were found growing on pine wood. Over the past 2 years, James and [...]]]></description>
				<content:encoded><![CDATA[<p><a class="post_image_link" href="http://amateurmycology.com/?p=1164" title="Permanent link to Working with Pine Loving Pleurotus"><img class="post_image alignleft frame" src="http://amateurmycology.com/wp-content/uploads/2012/05/pineoyster-e1337074971693.jpg" width="299" height="199" alt="pineoyster e1337074971693 Working with Pine Loving Pleurotus"  title="Working with Pine Loving Pleurotus" /></a>
</p><p>This quick update will cover our recent project with <a href="http://fds.duke.edu/db/aas/Biology/fungi" target="_blank">Dr. Rytas Vilgalys</a>, Professor of Biology at Duke University. Dr. Rytas was kind enough to do some <a href="http://en.wikipedia.org/wiki/Polymerase_chain_reaction" target="_blank">PCR DNA</a> and get an <a href="http://en.wikipedia.org/wiki/Internal_transcribed_spacer" target="_blank">ITS sequence</a> of some of our various collections of Pleurotus that were found growing on pine wood.</p>
<p>Over the past 2 years, James and I have been on the hunt for an oyster mushroom that grows on pine. To explain, I should first give a little background about these particular fungi. They tend to favor deciduous wood, especially poplar trees in Colorado. To most fungi, pine wood isn&#8217;t something easy to eat. Coniferous trees contain natural resins that tend to contain chemicals that are quite anti-fungal. This does not mean that fungi cannot eat coniferous wood, it is just quite difficult for them to do. However, Pleurotus are special fungi. They have discovered a quite efficient method of decomposing their substrate, excreting powerful digestive enzymes able to breakdown many different types of freshly cut wood. There is even <a href="http://www.fungaiolisiciliani.it/vari/foto%20Nicola%20Amalfi//Pleurotus_opuntiae.JPG" target="_blank">a type of Pleurotus that can grow on cactus skeletons</a>! The Pleurotus that James and I had found were growing from the cut stumps of pine trees and a spruce log growing at 10,000&#8242; in elevation. These were cultured in our lab in Denver and were eventually sent to Dr. Rytas. He was interested in them because of their unique habitats they were found growing in and was wondering, as we were, if they were a possible new species of Pleurotus. He also received a few control collections of <em>Pleurotus pulmonarius</em> from various locations in the state, growing from cottonwood trees. These were tested against the mushrooms that were found growing on conifers. This testing is basically done by taking known sets of genes from a specific species of fungi and testing samples of DNA against those genes to see if they are similar enough to be the same genetic group (hopefully that wasn&#8217;t too over simplified?). Much to our surprise, every specimen came back as <em>P. pulmonarius</em>! It is well documented that <em>P. pulmonarius</em> grows occasionally from conifer, but these specimens also had unique morphological features that were distinctly different, even with indoor grown mushrooms in a controlled environment! Just goes to show that morphology sometimes can tell you very little about what type of fungi you have. We now know that our cultures of oysters collected from these conifer habitats are P. pulmonarius, but have somehow developed a unique set of digestive enzymes to deal with the oils in the wood that tend to inhibit fungi.</p>
<p>The reason James and I are extremely interested in pine loving oysters is because the Rocky Mountains are plagued with a pine eating beetle that is destroying our forests by the entire mountainside. The general way to deal with this problem (due to the extreme fire danger it creates) is to cut down the infected trees and pile them in large piles. They are used mainly for fire wood, but the pine loving  mushrooms would decompose the wood much faster, creating larger soil depth. Paul Stamets has a discussion in his book, Mycelium Running, about the honey mushroom and its method of creating a deeper humus depth. Many of these plagues, whether they be a fungus or an insect, may look very disastrous to the environment to humans. This is only because of our perception of time. The trees that once stood as a forest are now decomposing, increasing the potential of the forest to create more life. As soil depth increases, the forest can house more and more plants. We would like to make these Pleurotus part of the solution to this large problem we are facing today.</p>
<p>&nbsp;</p>
]]></content:encoded>
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		</item>
		<item>
		<title>Written Report of RMC 2011</title>
		<link>http://amateurmycology.com/?p=1139</link>
		<comments>http://amateurmycology.com/?p=1139#comments</comments>
		<pubDate>Sun, 18 Sep 2011 20:51:09 +0000</pubDate>
		<dc:creator>Graham</dc:creator>
				<category><![CDATA[Events]]></category>
		<category><![CDATA[Featured Publications]]></category>
		<category><![CDATA[Green Mushroom Cultivation]]></category>
		<category><![CDATA[Mycorestoration]]></category>
		<category><![CDATA[Our 2011 Trips]]></category>

		<guid isPermaLink="false">http://amateurmycology.com/?p=1139</guid>
		<description><![CDATA[The following article is from radicalmycology.com and is a written report all about the recent Radical Mycology Convergence 2011 that was held in Concrete, WA in early September. Amateur Mycology attended the event giving presentations on &#8216;Green Mushroom Cultivation&#8217; and &#8216;Forest Floor Cultivation&#8217; techniques. Very soon we will have video clips of the event as [...]]]></description>
				<content:encoded><![CDATA[<p><a class="post_image_link" href="http://amateurmycology.com/?p=1139" title="Permanent link to Written Report of RMC 2011"><img class="post_image alignleft frame" src="http://amateurmycology.com/wp-content/uploads/2011/09/open-air-cultivation1-e1316380680702.jpg" width="300" height="200" alt="open air cultivation1 e1316380680702 Written Report of RMC 2011"  title="Written Report of RMC 2011" /></a>
</p><p>The following article is from radicalmycology.com and is a written report all about the recent Radical Mycology Convergence 2011 that was held in Concrete, WA in early September. Amateur Mycology attended the event giving presentations on &#8216;Green Mushroom Cultivation&#8217; and &#8216;Forest Floor Cultivation&#8217; techniques. Very soon we will have video clips of the event as well, here at amateurmycology.com, so stay tuned!</p>
<p><em><strong>-September 14th, 2011</strong>-</em></p>
<p><em> </em>Over 200 people gathered in northern Washington state this past Labor Day weekend to learn about the many uses of the fungal kingdom at the world’s first Radical Mycology Convergence. For four days, people gathered from several countries and various cultural backgrounds to teach and learn together about mycoremediation, the use of fungi as a tool to help combat mass pollution and ecological degradation. In an age when so many human caused disasters are occurring throughout the world, the fungi are beginning to be seen as a strong option for tackling some of these great problems long thought impossible to solve.</p>
<p><strong>WHY RADICAL MYCOLOGY?</strong></p>
<p>Access to mycological information is not easy. With a cultural view that fears fungi, a schooling system that undervalues them, and only a small number of courses on advanced mycology worldwide, it is easy to see why the fifth kingdom is so disregarded and misunderstood. As one of the youngest natural sciences, mycology (the study of fungi) has largely been kept in the hands of professionals since its development with much of the official work focusing simply on taxonomy and species edibility/toxicity. However, in the last few decades (and really just the last few years) the greater fungi have started to gain more acceptance and familiarity to those outside of academia as their uses beyond the dinner plate are starting to be realized.</p>
<p>It is surprising to note that most people do not realize that fungi are not only on, in and a part of all living (and once-living) things but that they play an extremely important role in the life cycle of plants as well. Acting like stewards of the forest, certain fungi create complex networks of “mycelium” (that white stuff you see when you pull back a decaying log) underground that serve to channel nutrients and water between plants and to help maintain the health of entire ecosystems. The fungi are also responsible for the decomposition of all woody material, turning dead plant matter in to fresh soil for new plants to thrive in. Without the fungi the world would be piled high in dead trees with no new ones growing.</p>
<p>In the last decade or so, mycologists have discovered that the same enzymes that fungi naturally produce to digest their food can also be used to break down toxic pollutants and petroleum products. Species have been discovered that can digest plastics, disposable diapers, motor oil, DDT, and Agent Orange as well as sequester and concentrate heavy metals out of polluted soil for later disposal. This emerging field of “mycoremediation” has only barely gained a foundation from which to grow on as in-depth research and experimentation in the last few years has been scant at best and suppressed at worst. As such a powerful ally in the fight to save the planet before ecological collapse, the fungi are now more worthy of investigation than ever before*. Thus, the RMC was formed to foster a community of people interested in developing and implementing mycoremediative techniques to provide a resource for peer learning and encouragement.</p>
<p>Through the use of fungi to enact change, we are attempting to radically challenge assumptions about the importance of the fungal kingdom in an effort to help shift our relationship to the Earth toward greater harmony.</p>
<p><strong>WHY A CONVERGENCE?</strong></p>
<p>The intent of the organizers of the RMC in forming the event was three fold: 1) To share mycological information in an accessible manner using the simplest techniques and a minimal amount of equipment 2) To promote the use of mycoremediation techniques &amp; 3) To build an all-inclusive &amp; non-hierarchical network of amateur &amp; professional mycologists. We feel we were quite successful in our efforts to a degree beyond any expectations.</p>
<p>Despite a full schedule all weekend, the RMC went off without a hitch. Workshops included sterile and non-sterile cultivation methods, mycopermaculture/mushrooms in the garden, mycomedicinals, mushroom paper and dye making, and fungi and lichen identification. There were also presentations on ethnomycology in Mexico by professional mycologists from Baja California. Folks from the Amazon Mycorenewal Project spoke on their work to clean up oil spills in Ecuador using oyster mushrooms. And a representative from the Mushroom Development Foundation spoke to their work teaching Indian farmers to grow mushrooms from agricultural waste. All this took place on a communal farm with nightly group fires, a raging talent show and raffle, and great swimming holes. Add in a general sense of commonality and you get an inspiring weekend of learning and building a community where one had not existed before.</p>
<p>Many presenters demonstrated techniques they had developed on their own to reduce the use of fossil fuels and expensive equipment from cultivating mushrooms. James from Amateur Mycology in Colorado stated that he hadn’t thrown away a piece of paper for 2 years as he was turning it all into mushrooms. James also spoke of successes in using mushroom beds as living mulch in a greenhouse to increase plant yields. Another workshop demonstrated tissue culturing in open air using only hydrogen peroxide and alcohol to sterilize your equipment. A big take away message from the weekend was that there is so much yet to be discovered about mycology–and so few people doing it–that it will take the work of amateurs to increase understanding.</p>
<p>As a culmination to the weekend, we implemented 2 small remediation projects at the host farm to put theory to practice. We set up 2 beds of King Stropharia mushrooms to help decompose the humanure produced at the farm. We also installed various burlap sacks inoculated with Blue Oyster mushrooms around the farm’s spring to help filter the water or possible runoff from a nearby road as well as prevent erosion to the surrounding hill side.</p>
<p>Through the RMC we created an environment that encouraged skill and knowledge sharing by embracing diversity and working toward the greater goal of a healthier planet and way of life. With the advances being made over the last few years, working with the fungi has never been easier than now, at a time when their capabilities are of greatest import. This information deserves to be in the hands of those who want it and the Radical Mycology Convergence was one step among several toward reaching that goal.</p>
<p><strong>NEXT STEPS</strong></p>
<p>On the final day of the convergence an open discussion was held to reflect on the RMC and to discuss ideas for future gatherings as well as how folks plan to implement this information in their local communities. The consensus showed that those present were excited to begin the process of developing a web-based forum or wiki to enable cultivators and experimenters to share techniques and experiences in relation to low-tech cultivation and remediation work. Similarly, free publications will be produced that teach these techniques and demonstrate case studies of the work people are doing with fungi. Also, a decentralized formal network will be created of groups of people doing this work so as to stay connected, organize future/regional RMCs, and to collaborate as desired.</p>
<p>A truly unique event, the first Radical Mycology Convergence was a huge success drawing in all types of people to live and learn together. The RMC demonstrated the power of a shared concern for the future of the planet to overcome personal differences in political or worldviews and the need to embrace novel ideas for tackling some of the world’s problems. We found that out of their backyards and garages, people are developing novel ways to work with the fungi to reduce their waste streams, filter their water, produce food and potent medicines easily, as well as work to clean up their local landbases thru remediation work.</p>
<p>The meme of radical mycology is only just developing. Time will tell how common this information and these techniques will become in the future. For now we invite those interested in learning more to follow the links and articles at <a href="http://www.radicalmycology.com/" target="_blank">www.radicalmycology.com</a>.</p>
<p>In sporidarity,</p>
<p><em>The Radical Mycology Convergence organizers</em></p>
<p><a href="mailto:radmycology@gmail.com">radmycology@gmail.com</a></p>
<p>* This is not to say this information addresses the problem of eliminating the manufacturing of these products. Rather it provides a way to actually deal with existing problems alongside efforts to stop their proliferation.</p>

<a href='http://amateurmycology.com/?attachment_id=1149' title='Open Air Cultivation- Jason Schindler '><img width="150" height="150" src="http://amateurmycology.com/wp-content/uploads/2011/09/open-air-cultivation-150x150.jpg" class="attachment-thumbnail" alt="open air cultivation 150x150 Written Report of RMC 2011"  title="Written Report of RMC 2011" /></a>
<a href='http://amateurmycology.com/?attachment_id=1148' title='Open Air Cultivation- Jason Schindler '><img width="150" height="150" src="http://amateurmycology.com/wp-content/uploads/2011/09/Open-Air-Cultivation-2-150x150.jpg" class="attachment-thumbnail" alt="Open Air Cultivation 2 150x150 Written Report of RMC 2011"  title="Written Report of RMC 2011" /></a>
<a href='http://amateurmycology.com/?attachment_id=1143' title='Andy MacKinnon on Lichens'><img width="150" height="150" src="http://amateurmycology.com/wp-content/uploads/2011/09/Andy-MacKinnon-on-lichens-150x150.jpg" class="attachment-thumbnail" alt="Andy MacKinnon on lichens 150x150 Written Report of RMC 2011"  title="Written Report of RMC 2011" /></a>
<a href='http://amateurmycology.com/?attachment_id=1145' title='Forest Floor Cultivation- Amateur Mycology'><img width="150" height="150" src="http://amateurmycology.com/wp-content/uploads/2011/09/Forest-Floor-Cultivation-AM-150x150.jpg" class="attachment-thumbnail" alt="Forest Floor Cultivation AM 150x150 Written Report of RMC 2011"  title="Written Report of RMC 2011" /></a>
<a href='http://amateurmycology.com/?attachment_id=1146' title='King Stropharia Humanure Project'><img width="150" height="150" src="http://amateurmycology.com/wp-content/uploads/2011/09/King-Stropharia-Humanure-Project-150x150.jpg" class="attachment-thumbnail" alt="King Stropharia Humanure Project 150x150 Written Report of RMC 2011"  title="Written Report of RMC 2011" /></a>
<a href='http://amateurmycology.com/?attachment_id=1150' title='RMC 2011 Talent Show'><img width="150" height="150" src="http://amateurmycology.com/wp-content/uploads/2011/09/talent-show-150x150.jpg" class="attachment-thumbnail" alt="talent show 150x150 Written Report of RMC 2011"  title="Written Report of RMC 2011" /></a>
<a href='http://amateurmycology.com/?attachment_id=1147' title='Mycelium Running'><img width="150" height="150" src="http://amateurmycology.com/wp-content/uploads/2011/09/mycelium-running-150x150.jpg" class="attachment-thumbnail" alt="mycelium running 150x150 Written Report of RMC 2011"  title="Written Report of RMC 2011" /></a>
<a href='http://amateurmycology.com/?attachment_id=1144' title='Bunker Spawn'><img width="150" height="150" src="http://amateurmycology.com/wp-content/uploads/2011/09/Bunker-Spawn-150x150.jpg" class="attachment-thumbnail" alt="Bunker Spawn 150x150 Written Report of RMC 2011"  title="Written Report of RMC 2011" /></a>

<p>PHOTOS BY Charlotte RMC</p>
<p>&nbsp;</p>
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		</item>
		<item>
		<title>Post RMC 2011 Interview</title>
		<link>http://amateurmycology.com/?p=1128</link>
		<comments>http://amateurmycology.com/?p=1128#comments</comments>
		<pubDate>Tue, 13 Sep 2011 18:19:23 +0000</pubDate>
		<dc:creator>Graham</dc:creator>
				<category><![CDATA[Events]]></category>
		<category><![CDATA[Featured Publications]]></category>
		<category><![CDATA[Green Mushroom Cultivation]]></category>
		<category><![CDATA[Mycorestoration]]></category>
		<category><![CDATA[Our 2011 Trips]]></category>
		<category><![CDATA[Amazon Mycorenewal Project]]></category>
		<category><![CDATA[asia]]></category>
		<category><![CDATA[cancer]]></category>
		<category><![CDATA[community farm]]></category>
		<category><![CDATA[Concrete]]></category>
		<category><![CDATA[connecting people]]></category>
		<category><![CDATA[connection to nature]]></category>
		<category><![CDATA[cultivation]]></category>
		<category><![CDATA[dyeing]]></category>
		<category><![CDATA[entheogen]]></category>
		<category><![CDATA[entheogenic]]></category>
		<category><![CDATA[fungi]]></category>
		<category><![CDATA[ganoderma]]></category>
		<category><![CDATA[KOWA]]></category>
		<category><![CDATA[lucidium]]></category>
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		<category><![CDATA[mycofiltration]]></category>
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		<category><![CDATA[mycomedicinals]]></category>
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		<category><![CDATA[mycorestoration]]></category>
		<category><![CDATA[oil spills]]></category>
		<category><![CDATA[old growth forest]]></category>
		<category><![CDATA[papermaking]]></category>
		<category><![CDATA[pollution]]></category>
		<category><![CDATA[presentations]]></category>
		<category><![CDATA[Radical Mycology Convergence]]></category>
		<category><![CDATA[recycling]]></category>
		<category><![CDATA[reishi]]></category>
		<category><![CDATA[saving the environment]]></category>
		<category><![CDATA[sterile cultivation]]></category>
		<category><![CDATA[sustainable]]></category>
		<category><![CDATA[the fifth kingdom]]></category>
		<category><![CDATA[uses of fungi]]></category>
		<category><![CDATA[WA]]></category>
		<category><![CDATA[Washington State]]></category>
		<category><![CDATA[workshops]]></category>

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		<description><![CDATA[James and I just got back on the 7th of September from the Radical Mycology Convergence 2011. One of the organizers of the event got on the local short wave radio station, KOWA, in Olympia, WA yesterday to talk about the RMC and plans for the future. Check out the interview below. A written report [...]]]></description>
				<content:encoded><![CDATA[<p><a class="post_image_link" href="http://amateurmycology.com/?p=1128" title="Permanent link to Post RMC 2011 Interview"><img class="post_image alignleft frame" src="http://amateurmycology.com/wp-content/uploads/2011/09/RMC-e1316380759695.jpg" width="300" height="142" alt="RMC e1316380759695 Post RMC 2011 Interview"  title="Post RMC 2011 Interview" /></a>
</p><p>James and I just got back on the 7th of September from the Radical Mycology Convergence 2011. One of the organizers of the event got on the local short wave radio station, KOWA, in Olympia, WA yesterday to talk about the RMC and plans for the future. Check out the interview below. A written report of the RMC to come soon. Stay tuned.</p>
<p>&nbsp;</p>
<p>Part 1:<br />
<iframe src="http://www.youtube.com/embed/_KrBs0ZtSp8" frameborder="0" width="640" height="390"></iframe></p>
<p>&nbsp;</p>
<p>Part 2:<br />
<iframe src="http://www.youtube.com/embed/7_2EiOvA5ZY" frameborder="0" width="640" height="390"></iframe></p>
<p>&nbsp;</p>
<p>Part 3:<br />
<iframe src="http://www.youtube.com/embed/Y0U7z7JRtUw" frameborder="0" width="640" height="390"></iframe></p>
<p>Visit <a href="http://radicalmycology.wordpress.com/" target="_blank">http://radicalmycology.wordpress.com/</a> for more information!!</p>
]]></content:encoded>
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		<title>Looking for a quick introduction to mycorestoration?</title>
		<link>http://amateurmycology.com/?p=47</link>
		<comments>http://amateurmycology.com/?p=47#comments</comments>
		<pubDate>Fri, 15 Oct 2010 06:24:01 +0000</pubDate>
		<dc:creator>Graham</dc:creator>
				<category><![CDATA[Green Mushroom Cultivation]]></category>
		<category><![CDATA[Mycorestoration]]></category>
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		<category><![CDATA[Paul Stamets]]></category>
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		<description><![CDATA[Paul Stamets, author of &#8216;Mycelium Running: How Mushrooms Can Save The World&#8217;. He is obviously one of the smartest people on earth. Everyone should see this quick TED.com video, plus if you haven&#8217;t already, check out the other videos they have. All of the ones I have watched are amazing. Also, check out Paul&#8217;s You [...]]]></description>
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<p>Paul Stamets, author of &#8216;Mycelium Running: How Mushrooms Can Save The World&#8217;. He is obviously one of the smartest people on earth. Everyone should see this quick TED.com video, plus if you haven&#8217;t already, check out the other videos they have. All of the ones I have watched are amazing. Also, check out  <a href="http://www.youtube.com/paulstamets">Paul&#8217;s You Tube Site</a> for his latest short videos!</p>
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